Kedu otu esi ahọrọ ngwa ahịa PCR/qPCR?

PCR bụ usoro dị nro ma dị irè maka ịkwalite otu nnomi nke usoro DNA lekwasịrị anya na nde nde n'ime obere oge. Ya mere, ihe eji eji plastik eme ihe maka mmeghachi omume PCR ga-abụ nke na-enweghị mmerụ na ndị na-egbochi ya, ebe ha nwere àgwà dị elu nke nwere ike ikwe nkwa mmetụta PCR kacha mma. PCR plastic consumables dị n'ụdị dị iche iche nke nha na usoro, na ịmara àgwà kwesịrị ekwesị nke ngwaahịa ga-enyere gị aka na-ahọpụta ndị ziri ezi plastic consumable maka ezigbo PCR na qPCR data.

Ngwakọta na njirimara nke ngwa PCR

1. Akụrụngwa
A na-ejikarị polypropylene eme ihe PCR, bụ nke na-adịghị mma iji guzogide mgbanwe okpomọkụ ngwa ngwa n'oge ịgba ígwè ọkụ ma belata ihe ndị na-emeghachi omume iji hụ na nsonaazụ PCR kacha mma. ahụike-ọkwa, elu-edu polypropylene akụrụngwa kwesịrị iji n'oge mmepụta na arụpụtara na klaasị 100,000 cleanroom. Ngwaahịa a ga-enwere onwe ya pụọ ​​​​na nuclease na mmetọ DNA iji zere itinye aka na mmetụta nke nnwale mmụba DNA.

2.Agba
PCR eferenaPCR tubesdị n'ozuzu dị na uzo na ọcha.

• The edo mgbidi ọkpụrụkpụ imewe ga-agbanwe agbanwe okpomọkụ nyefe maka reacting samples.

• Ogo ngwa anya dị elu iji hụ na mgbasa ozi fluorescence kacha mma yana obere mgbagha.

• Na nyocha qPCR, oghere ọcha ahụ gbochiri ntugharị nke mgbaàmà fluorescence na nnabata ya site na modul kpo oku.

3.Format
The PCR Plate "uwe elu" dị gburugburu osisi. Uwe mwụda ahụ na-enye nkwụsi ike ka mma maka usoro pipetting mgbe arụ ọrụ mmeghachi omume na-arụ ọrụ, ma na-enye ike ọrụ dị mma n'oge usoro ọgwụgwọ akpaka. Enwere ike kewaa efere PCR n'ime enweghị uwe mwụda, uwe mwụda ọkara na uwe mwụda zuru oke.

• Efere PCR na-abụghị skirted na-efu n'akụkụ efere ahụ, na ụdị mmeghachi omume a nwere ike imegharị maka ọtụtụ ngwa PCR na ngwa ngwa PCR ozugbo, mana ọ bụghị maka ngwa akpaaka.

• Plate PCR nke nwere ọkara nwere obere mkpirisi n'akụkụ nsọtụ efere ahụ, na-enye nkwado zuru oke n'oge pipetting na ike igwe maka njikwa roboti.

• Efere PCR zuru oke nwere ọnụ nke na-ekpuchi elu efere ahụ. Ụdị efere a dabara adaba maka arụ ọrụ akpaaka, nke nwere ike ịdị mma ma kwụsie ike. Uwe uwe mwụda zuru oke na-emekwa ka ike igwe rụọ ọrụ, na-eme ka ọ dị mma maka iji robots na-arụ ọrụ na-akpaghị aka.

PCR tube dị na otu na 8-strips tube, nke dabara adaba maka nnwale PCR/qPCR dị ala na nke ọkara. Emebere mkpuchi ahụ dị larịị iji kwado ide ihe, yana nnyefe ntụkwasị obi dị elu nke mgbaàmà fluorescence nwere ike ime nke ọma site na qPCR.

• Otu tube na-enye mgbanwe iji tọọ ọnụ ọgụgụ mmeghachi omume kpọmkwem. Maka mpịakọta mmeghachi omume buru ibu, otu tube dị na nha 0.5 ml dị.
• The 8-strips tube nwere okpu na-emeghe ma na-emechi tubes sample onwe ha iji gbochie sample.

4.akara
Ihe mkpuchi tube na ihe nkiri mkpuchi ga-emechicha tube na efere iji gbochie evaporation nke sample n'oge okpomọkụ okirikiri. Enwere ike nweta akara siri ike site na iji ihe nchacha ihe nkiri na ngwá ọrụ pịa.

• Olulu mmiri PCR nwere akụkụ dị elu gburugburu ha. Nhazi a na-enyere aka mechie efere ahụ na ihe nkiri mkpuchi iji gbochie evaporation.
• Akara mkpụrụedemede na efere PCR ga-enyere aka ịchọpụta olulu mmiri nke ọ bụla na ọnọdụ nke ihe atụ kwekọrọ. A na-ebikarị mkpụrụ akwụkwọ mgbịrịgba na-acha ọcha ma ọ bụ oji, na maka ngwa akpaghị aka, mkpụrụedemede na-aba uru maka ịchichi akụkụ mpụta nke efere ahụ.

5.Flux ngwa

Mmụgharị nnwale nke nyocha PCR / qPCR nwere ike ikpebi ụdị ngwaahịa rọba ga-eji maka nsonaazụ ọgwụgwọ kacha mma. Maka ngwa ntinye nke dị ala na-agafeghị oke, tubes na-adịkarị mma karịa, ebe efere na-achọsi ike maka nnwale nke ọkara ruo elu. A na-emepụtakwa efere iji tụlee mgbanwe mgbanwe nke flux, nke nwere ike kewaa n'ime otu eriri.

Na mmechi, dị ka akụkụ dị mkpa nke PCR usoro iwu, plastic consumables dị oké mkpa maka ịga nke ọma nke nnwale na nchịkọta data, karịsịa na ọkara-na-elu throughput workflow ngwa.

Dị ka ndị China na-eweta ihe eji eji plastik akpaaka, Cotaus na-enye ndụmọdụ pipette, nucleic acid, nyocha protein, ọdịbendị cell, nchekwa ihe nlele, akara, chromatography, wdg.

Pịa aha ngwaahịa iji lelee nkọwa ngwaahịa PCR na-eri.

PCR tube ;PCR efere