Mbata ọhụrụ | ire | Black Elisa efere

N'ọnọdụ dị iche iche na sayensị ndụ, mkpebi nke oge, nke ọma na nke akụ na ụba na ọnụọgụ nke antigens ma ọ bụ ọgwụ mgbochi dị na ihe nlele bụ ihe dị mkpa.

Enzyme-linked immunosorbent assay (ELISA) egosila na ọ bụ nchọpụta bara uru na ngwaọrụ nyocha maka ịlele ọgwụ mgbochi ma ọ bụ antigens na ihe atụ nke ndụ site na ntinye nke antigens ma ọ bụ ọgwụ mgbochi a maara n'elu elu nke onye na-ebu ihe siri ike, nke na-enye ohere maka enzyme. Nke kariri HRP) - nke akpọrọ antigen-antibody mmeghachi omume n'elu ala siri ike. Enwere ike iji usoro a chọpụta nnukwu antigens molecule na mgbochi mgbochi kpọmkwem, wdg. Otú ọ dị, mmetụta uche na ike dị iche iche nke nchọpụta ELISA na-ejedebe nke ukwuu site na adịghị ike nke usoro absorption ọkụ n'ihi mmetụta dị ukwuu nke ọnọdụ mpụga na mgbanwe agba nke ngwọta na ọnụ ala dị ala nke linear nke uru OD.

Teknụzụ DELFIA ---- bụ naanị iji lanthanide chelate dochie enzyme HRP (Eu, Sm, Tb, Dy) na-edobe na mgbochi nchọpụta na nyocha ELISA ọdịnala. Lanthanides eji na DELFIA bụ klaasị pụrụ iche nke fluorescent ọcha, nke na-etinye ihe a chọrọ na ihe nnwale --- efere Elisa. Lanthanides nwere ogologo oge ndụ nke microseconds ma ọ bụ ọbụna milliseconds, nke jikọtara ya na nchọpụta edozichara oge na-ebelata nnyonye anya ndabere autofluorescence, na mgbanwe Strokes buru ibu ha na-eme ka nghọta nke nyocha ahụ dịkwuo mma.

Ọnụ ọgụgụ ka ukwuu nke ELISA na-ahọrọ efere ntinye aha enzyme transperent dị ka onye na-ebu na akpa, mana ọkụ na-apụta na mmeghachi omume luminescence bụ isotropic, ìhè agaghị agbasasị site na vetikal ntụziaka, ma na-agbasasịkwa si kehoraizin direction, na ọ ga- dị mfe na-agafe oghere dị n'etiti oghere dị iche iche nke mpempe akwụkwọ ntinye aha enzyme transperent na mgbidi nke oghere. Oghere ndị agbata obi na-ejikọta ibe ha ma na-emetụta nsonaazụ nnwale.

Enwere ike iji efere Elisa White maka nchọpụta ọkụ na-esighị ike ma na-ejikarị eme ihe maka chemiluminescence izugbe na mmepe ụcha mkpụrụ (dịka dual luciferase reporter gene analysis).

Plate Elisa White White nwere mgbaama na-esighi ike karịa efere nke na-ede aha enzyme ọcha n'ihi mmịnye ọkụ nke ha, a na-ejikwa ya maka ịchọpụta ọkụ siri ike, dị ka nchọpụta fluorescence.

Uru nke Cotaus®Elisa Plates

● Njikọ dị elu

A na-eme efere Cotaus®Elisa nwere tube ojii nke ihe na-abụghị nke onwe ya, a na-agwọkwa elu ya ka ọ dịkwuo ike ijikọ protein ya, nke nwere ike iru 500ng IgG / cm2, na ịdị arọ nke molekul nke protein protein bụ> 10kD. .

● Igwe ọkụ n'azụ dị ala na-ewepụ nsogbu ndị mmeghachi omume na-enweghị isi kpatara.

tubs ojii nwere ike iwepụ ụfọdụ fluorescence nnyonye anya ndabere na-esighi ike n'ihi na ọ ga-enwe nnabata ọkụ nke ya.

● Ekewapụ ihe

Nhazi nke nwere ike ịwepụ nke etiti efere enzyme ọcha na slats ojii enzyme dị mma maka ịrụ ọrụ. Lezienụ anya na ihe ndọsa, amanyela imebi n'otu njedebe, ma ọ bụghị ya, ọ ga-adị mfe ịgbaji.

Nhazi ngwaahịa

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