Kedu ihe kpatara eji PP na-ejikarị eme ihe PCR?

"Dịka anyị niile maara, PCR bụ usoro nnwale bụ isi na ụlọ nyocha biochemical." Nsonaazụ nnwale a na-adịghị mma mgbe niile, nke nwere ike ịbụ n'ihi ntakịrị mmetọ nke PCR plastic consumable, ma ọ bụ nnyonye anya nnwale kpatara site na iwebata ihe mgbochi. Enwere ihe ọzọ dị oke mkpa: nhọrọ na-ezighị ezi nke ihe oriri ga-enwekwa mmetụta dị ukwuu na nsonaazụ nnwale.

Enwere ọtụtụ ihe kpatara na-emetụta nsonaazụ PCR: na-enwekarị ụdị 7 ndị a.

1. Primers: Primers bụ isi ihe maka mmeghachi omume kpọmkwem nke PCR, na njirimara nke ngwaahịa PCR na-adabere n'ókè nke mmeju n'etiti primers na DNA template;

2. Enzyme na ntinye ya;

3. Ogo na ntinye uche nke dNTP;

4. Template (content gene) nucleic acid;

5. Mg2+ itinye uche;

6. Ntọala okpomọkụ na oge;

7. Ọnụ ọgụgụ nke okirikiri;

8. Akụrụngwa, ihe oriri, wdg.

N'ime ọtụtụ ihe ndị na-emetụta, ihe oriri bụ otu n'ime ihe ndị dị oke mkpa na nke a na-eleghara anya.

Enwere ọtụtụ ụdịPCR ihe eji eme ihe: 8-tube, tubes dị ala, tubes ọkọlọtọ, ndị na-abụghị uwe, ọkara-skirted, zuru oke, na usoro PCR na qPCR efere. O siri ike ịhọrọ, ma enwere ọtụtụ nsogbu ndị a na-ahụkarị, ka anyị leba anya na nsogbu nke onye ọ bụla na-ahọrọPCR ihe eji eme ihe, na otu esi edozi ha?

Gịnị merePCR ihe eji eme ihen'ozuzu mere nke PP?

Azịza: PCR / qPCR consumables bụ n'ozuzu mere nke polypropylene (PP), n'ihi na ọ bụ a biologically inert ihe onwunwe, n'elu adịghị mfe n'ịgbaso biomolecules, na nwere ezi chemical iguzogide na okpomọkụ ndidi (nwere ike autoclaved na 121 degrees) bacteria. ma nwee ike iguzogide mgbanwe okpomọkụ n'oge ịgba ígwè ọkụ). Ihe ndị a na-emekarị na kọntaktị na reagents ma ọ bụ ihe nlele, ya mere ọ dị mkpa ka ịhọrọ ihe ndị dị elu na usoro nhazi dị mma n'oge mmepụta na usoro nkwadebe.