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Ngwa a na-ahụkarị nke Plate ELISA

2024-06-12

Dị ka ngwá ọrụ nnwale, isi nhazi nkeEfere ELISAbụ usoro nke microplates nwere ihe siri ike nke akụkụ (dị ka protein na mgbochi). N'ime ngwa nke efere ELISA, ihe nlele a ga-anwale ga-emeghachi omume na mkpụrụ ndụ ihe nketa nke enzyme a kapịrị ọnụ, mgbe ahụ, a ga-emepụta mgbanwe agba a na-ahụ anya site na ịgbakwunye mkpụrụ osisi matrix, na ọdịnaya ma ọ bụ ọrụ nke molecule lekwasịrị anya ga-atụle. ma ọ bụ enyocha ya site n'ịchọpụta mgbama nnabata ma ọ bụ fluorescence. Ihe ndị a bụ ngwa nkịtị nke efere ELISA na mpaghara dị iche iche:

1. Protein quantitative analysis: E nwere ike iji efere ELISA tụọ itinye uche na ọrụ nke ndị na-edozi ahụ na ihe atụ nke ndụ dị ka serum na cell supernatants, na-enye ngwá ọrụ dị ike maka nchọpụta ihe nrịbama tumor, nje nje nje ịba ọcha n'anya, ihe nrịbama myocardial, wdg. na inyere ndị dọkịta aka n'oge nyocha na nyocha nke ọrịa.

2. Nleba anya nke cytokine: N'ime nyocha nke immunoology,Efere ELISAnwere ike ịlele ọkwa cytokine na ndị na-ahụ maka omenala sel ma ọ bụ mmiri anụ ahụ, nke na-enyere aka ịghọta usoro ihe ndị dị ndụ dị ka nzaghachi mgbochi na nzaghachi mkpali, ọ dịkwa oke mkpa maka mmepe nke ọgwụgwọ na ọgwụ ọhụrụ.

3. Nchọpụta Nucleic acid: Site na efere ELISA, ndị ọkà mmụta sayensị nwere ike ịchọpụta na nyochaa ọdịnaya na ọrụ nke DNA ma ọ bụ RNA, nye nkwado data maka nchọpụta mkpụrụ ndụ ihe nketa dị ka okwu mkpụrụ ndụ ihe nketa na nhazi mkpụrụ ndụ, ma na-akwalite mmepe nke ubi dịka ọgwụgwọ mkpụrụ ndụ ihe nketa. na edezi mkpụrụ ndụ ihe nketa.

4. Nnyocha ọrụ Enzyme: Efere ELISA nwere ike tụọ ọrụ enzyme n'ụzọ ziri ezi, nyere ndị na-eme nchọpụta aka ịghọta ọrụ na usoro nhazi nke enzymes n'ime ihe ndị dị ndụ, ma nye ntụaka dị mkpa maka nyocha na enzyme engineering, metabolic engineering na ubi ndị ọzọ.

5. Nnyocha mmekọrịta nke intermolecular:Efere ELISAenwere ike iji ọ bụghị naanị iji tụọ ọdịnaya nke ụmụ irighiri ihe, kamakwa iji mụọ mmekọrịta dị n'etiti ụmụ irighiri ihe. Site na ijikọta teknụzụ dị ka plasmon resonance elu na fluorescence resonance energy transfer, usoro njikọ na nkewa n'etiti ụmụ irighiri ihe nwere ike nyochaa ozugbo, na-enye echiche ọhụrụ na ụzọ maka imepụta ọgwụ, mmekọrịta protein na nyocha ndị ọzọ.

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